recombinant human stem cell factor (hscf) (PeproTech)

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PeproTech recombinant human stem cell factor (hscf)
Recombinant Human Stem Cell Factor (Hscf), supplied by PeproTech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/recombinant+human+stem+cell+factor+(hscf/recombinant+human+stem+cell+factor/pmc11321652-127-27-32
Average 90 stars, based on 1 article reviews

recombinant human stem cell factor (hscf) - by Bioz Stars, 2026-09

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Article Title: Library‐Assisted Evolution in Eukaryotic Cells Yield Adenine Base Editors with Enhanced Editing Specificity
Article Snippet: Human CD34 + cells were cultured in X‐VIVO 15 (04‐418Q, Lonza) supplemented with 100 ng mL −1 recombinant human thrombopoietin (PeproTech, AF‐300‐18), 100 ng mL −1 recombinant human stem cell factor (hSCF) (PeproTech, AF‐300‐07) and 100 ng mL −1 recombinant human Flt3‐ligand (PeproTech, AF‐300‐19).

Article Title: CRISPR Editing Enables Consequential Tag-Activated MicroRNA-Mediated Endogene Deactivation
Article Snippet: HUDEP-2 cells were expanded in medium based on StemSpan SFEM II (Stem Cell Technologies, Vancouver, BC, Canada) supplemented with 1 μM Dexamethasone (Sigma-Aldrich, Munich, Germany), 5 μg/mL Doxycycline (Clontech Laboratories, Mountain View, CA, USA), 100 ng/mL Recombinant Human Stem Cell Factor (hSCF) (PeproTech, Rocky Hill, CT, USA), 3 IU/mL Epoetin alpha (Binocrit 4000 IU/0.4 mL, Sandoz GmbH, Kundl, Austria), and 2× Penicillin-Streptomycin (Thermo Fisher Scientific, Waltham, MA, USA), at concentrations below 0.5 × 10 6 cells/mL, following procedures for expansion and erythroid differentiation in a three-phase erythroid differentiation culture system as previously described [ ].

Article Title: Scalable generation of functional human iPSC-derived CAR-macrophages that efficiently eradicate CD19-positive leukemia.
Article Snippet: CD34+ cells were cultured in StemSpan medium (STEMCELL Technologies, Vancouver, Canada) containing 1 mM penicillin–streptomycin (P/S), 2 mM L- glutamine (L- Glu), 100 ng/mL human stem cell factor (hSCF), 100 ng/mL FMS- like tyrosine kinase3 ligand (hFlt3L) and 50 ng/mL human thrombopoietin (hTPO) (PeproTech, Rocky Hill, USA), further details can be found in the previous published work.14 Transduction of cord blood-derived CD34+ cells and differentiation to macrophages 24 hours post- isolation, 2×105 freshly- isolated cord bloodderived CD34+ cells were transduced with a multiplicity of infection (MOI) of 5 and further sorted and differentiated into mature macrophages as shown in detail in a previous study.14 iPS cell cultivation and differentiation to macrophages iPSC cultivation was performed using a feeder- free protocol. iPSC cells were cultured on Geltrex- coated tissue culture plates or T25 flasks using E8 medium (containing DMEM/F- 12 (Gibco, Life Technologies), 64 mg/L ascorbic acid 2- phosphate, 14 μg/L sodium selenite, 543 mg/L, NaHCO3 and 20 mg/L insulin, and 10,7 mg/L human recombinant transferrin (all from SigmaAldrich) supplemented with 100 ng/mL human basic fibroblast growth factor (hbFGF) and 2 ng/mL human transforming growth factor beta (TGFß) (both from PeproTech) under standard humidified conditions at 37°C and 5% CO2.

Article Title: CRISPR Editing Enables Consequential Tag-Activated MicroRNA-Mediated Endogene Deactivation.
Article Snippet: HUDEP-2 cells were expanded in medium based on StemSpan SFEM II (Stem Cell Technologies, Vancouver, BC, Canada) supplemented with 1 μM Dexamethasone (SigmaAldrich, Munich, Germany), 5 μg/mL Doxycycline (Clontech Laboratories, Mountain View, CA, USA), 100 ng/mL Recombinant Human Stem Cell Factor (hSCF) (PeproTech, Rocky Hill, CT, USA), 3 IU/mL Epoetin alpha (Binocrit 4000 IU/0.4 mL, Sandoz GmbH, Kundl, Austria), and 2× Penicillin-Streptomycin (Thermo Fisher Scientific, Waltham, MA, USA), at concentrations below 0.5 × 106 cells/mL, following procedures for expansion and erythroid differentiation in a three-phase erythroid differentiation culture system as previously described [37].

Article Title: Lactic acid suppresses MRGPRX2 mediated mast cell responses.
Article Snippet: Recombinant human stem cell factor (hSCF) and mouse stem cell factor (mSCF) was purchased from PeproTech (Rocky Hill, NJ, United States).

Article Title: Demethylating therapy increases anti-CD123 CAR T cell cytotoxicity against acute myeloid leukemia
Article Snippet: For primary AML or HD cells, the culture medium was additionally supplemented with 5 ng/mL recombinant human stem cell factor (hSCF) (Peprotech, Rocky Hill, NJ).

Article Title: Library-Assisted Evolution in Eukaryotic Cells Yield Adenine Base Editors with Enhanced Editing Specificity.
Article Snippet: Human CD34 + cells were cultured in X-VIVO 15 (04-418Q, Lonza) supplemented with 100 ng mL−1 recombinant human thrombopoietin (PeproTech, AF300-18), 100 ng mL−1 recombinant human stem cell factor (hSCF) (PeproTech, AF-300-07) and 100 ng mL−1 recombinant human Flt3-ligand (PeproTech, AF-300-19).

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Article Title: Identification and characterization of RBM12 as a novel regulator of fetal hemoglobin expression
Article Snippet: .. HUDEP2 cells were maintained in StemSpan SFEM (StemCell Technologies, #09650) supplemented with 50ng/ml recombinant human stem cell factor (hSCF, Peprotech, #300-07), 1uM dexamethasone (Sigma, #D4902), 1ug/ml doxycycline (Sigma, #D9891), 3IU/ml erythropoietin (EPO, Amgen, #55513-144-10), and 1% penicillin/streptomycin (ThermoFisher Scientific, #15140122) at a density of 100,000 – 1,000,000 cells/ml. .. Cells were differentiated for 5-7 days in Iscove’s Modified Dulbecco’s Medium (IMDM, Mediatech, #MT10016CV) supplemented with 50ng/ml hSCF, 3IU/ml EPO, 1ug/ml doxycycline, 5% fetal bovine serum, 320 ug/ml holo-transferrin (Sigma, #T4132), 10ug/ml insulin (Sigma, #I9278), and 10ug/ml heparin (Sigma, #H3149) at a density of 500,000-2,000,000 cells/ml.

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recombinant human stem cell factor (hscf) (PeproTech)

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